A promising diagnostic tool for toxoplasmic encephalitis: tachyzoite/bradyzoite stage-specific RT-PCR

Toxoplasmic Encephalitis

Development of multiplex real-time RT-PCR as a diagnostic tool for avian influenza.

A multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) assay for the simultaneous detection of the H5 and H7 avian influenza hemagglutinin (HA) subtypes was developed with hydrolysis type probes labeled with the FAM (H5 probe) and ROX (H7 probe) reporter dyes. The sensitivity of the H5-H7 subtyping assay was determined, using in vitro transcribed RNA templates, to have ...

Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR.

A rapid, sensitive and highly specific detection method for Aquareovirus based on reverse-transcription polymerase chain reaction (RT-PCR) was developed. Based on multiple sequence alignment of the cloned sequences of a local isolates, the Threadfin reovirus (TFV) and Guppy reovirus (GPV) with Grass carp reovirus (GCRV), a pair of degenerate primers was selected carefully and synthesized. Using...

Development of mRNA-specific RT-PCR for the detection of koi herpesvirus (KHV) replication stage.

An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR di...

Written Text as a Promising Diagnostic Tool for Parkinson’s Disease

In this particular case, we do not need to go abroad to witness a promising discovery. We can stay in Brno. At the Faculty of Electrical Engineering and Communication, Brno University of technology, a research team led by Jiří Mekyska is developing a tool for early diagnostics of Parkinson’s disease which is based on processing the handwriting and speech utterances of patients. And it has to be...